View source for 1z90

[[Image:1z90.gif|left|200px]]<br /><applet load="1z90" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1z90, resolution 1.86&Aring;" />
'''X-ray structure of gene product from arabidopsis thaliana at3g03250, a putative UDP-glucose pyrophosphorylase'''<br />

==Overview==
The structure of the UDP-glucose pyrophosphorylase encoded by Arabidopsis, thaliana gene At3g03250 has been solved to a nominal resolution of 1.86 A., In addition, the structure has been solved in the presence of the, substrates/products UTP and UDP-glucose to nominal resolutions of 1.64 A, and 1.85 A. The three structures revealed a catalytic domain similar to, that of other nucleotidyl-glucose pyrophosphorylases with a, carboxy-terminal beta-helix domain in a unique orientation. Conformational, changes are observed between the native and substrate-bound complexes. The, nucleotide-binding loop and the carboxy-terminal domain, including the, suspected catalytically important Lys360, move in and out of the active, site in a concerted fashion. TLS refinement was employed initially to, model conformational heterogeneity in the UDP-glucose complex followed by, the use of multiconformer refinement for the entire molecule. Normal mode, analysis generated atomic displacement predictions in good agreement in, magnitude and direction with the observed conformational changes and, anisotropic displacement parameters generated by TLS refinement. The, structures and the observed dynamic changes provide insight into the, ordered mechanism of this enzyme and previously described oligomerization, effects on catalytic activity.

==About this Structure==
1Z90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z90 OCA]. 

==Reference==
Structure and Dynamics of UDP-Glucose Pyrophosphorylase from Arabidopsis thaliana with Bound UDP-Glucose and UTP., McCoy JG, Bitto E, Bingman CA, Wesenberg GE, Bannen RM, Kondrashov DA, Phillips GN Jr, J Mol Biol. 2007 Feb 23;366(3):830-41. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17178129 17178129]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Allard, S.T.M.]]
[[Category: Bingman, C.A.]]
[[Category: Bitto, E.]]
[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]
[[Category: Jr., G.N.Phillips.]]
[[Category: Wesenberg, G.E.]]
[[Category: at3g03250]]
[[Category: center for eukaryotic structural genomics]]
[[Category: cesg]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: putative udp-glucose pyrophosphorylase]]
[[Category: structural genomics]]
[[Category: unknown function]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:16:50 2008''