View source for 1bsp

[[Image:1bsp.gif|left|200px]]<br />
<applet load="1bsp" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1bsp, resolution 2.50&Aring;" />
'''THERMOSTABLE THYMIDYLATE SYNTHASE A FROM BACILLUS SUBTILIS'''<br />

==Overview==
Unlike all other organisms studied to date, Bacillus subtilis expresses, two different thymidylate synthases: bsTS-A and bsTS-B. bsTS-A displays, enhanced enzymatic and structural thermal stability uncharacteristic of, most TSs. Despite the high level of TS conservation across most species, bsTS-A shares low sequence identity (&lt;40%) with the majority of TSs from, other organisms. This TS and the TSs from Lactococcus lactis and phage, Phi3T-to which it is most similar-have been of interest for some time, since, by structure-based sequence alignment, they appear to lack several, key residues shown by mutagenesis to be essential to enzymatic function, [Greene, P. J., Yu, P. L., Zhao, J., Schiffer, C. A., and Santi, D. (1994), Protein Sci. 3, 1114-6]. In addition, bsTS-A demonstrates ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9778348 (full description)]]

==About this Structure==
1BSP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with PO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Structure known Active Sites: CTA and CTB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BSP OCA]]. 

==Reference==
Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis., Stout TJ, Schellenberger U, Santi DV, Stroud RM, Biochemistry. 1998 Oct 20;37(42):14736-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9778348 9778348]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Thymidylate synthase]]
[[Category: Santi, D.V.]]
[[Category: Schellenberger, U.]]
[[Category: Stout, T.J.]]
[[Category: Stroud, R.M.]]
[[Category: PO4]]
[[Category: dtmp synthase]]
[[Category: methyltransferase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:41:34 2007''