View source for 2fl1

[[Image:2fl1.jpg|left|200px]]<br /><applet load="2fl1" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2fl1, resolution 2.4&Aring;" />
'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''<br />

==Overview==
The three-dimensional structure of the red fluorescent protein (RFP), zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric, unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in, space group C222(1). The crystal structure, refined to a crystallographic, R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed, of 11 strands similar to that of the yellow fluorescent protein zYFP538., The zRFP574 chromophore, originating from the protein sequence, Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins., The bond geometry of residue 66 shows the strong tendency of the, corresponding C(alpha) atom to sp(2) hybridization as a consequence of, N-acylimine bond formation. The zRFP574 chromophore contains the 65-66, cis-peptide bond characteristic of red fluorescent proteins. The, chromophore phenolic ring adopts a cis conformation coplanar with the, imidazolinone ring. The crystallographic study has revealed an unexpected, chemical feature of the internal chromophore. A decarboxylated side chain, of the chromophore-forming residue Asp66 has been observed in the, structure. This additional post-translational modification is likely to, play a key role in the bathochromic shift of the zRFP574 spectrum.

==About this Structure==
2FL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL1 OCA]. 

==Reference==
Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16627946 16627946]
[[Category: Single protein]]
[[Category: Zoanthus sp.]]
[[Category: Martynov, V.]]
[[Category: Pletnev, S.]]
[[Category: Pletnev, V.]]
[[Category: Pletneva, N.]]
[[Category: Popov, B.]]
[[Category: Tikhonova, T.]]
[[Category: SO4]]
[[Category: beta barrel]]
[[Category: beta-can fold]]
[[Category: button polyp]]
[[Category: chromophore]]
[[Category: crystal structure]]
[[Category: emission maximum 574nm]]
[[Category: fluorescent marker]]
[[Category: intersubunit interface]]
[[Category: red fluorescent protein]]
[[Category: tightly packed tetramer]]
[[Category: zoanthus sp.]]
[[Category: zrfp574]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:39:00 2008''