View source for 2ji8

[[Image:2ji8.gif|left|200px]]<br />
<applet load="2ji8" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2ji8, resolution 2.15&Aring;" />
'''X-RAY STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH FORMYL-COA'''<br />

==Overview==
Despite more than five decades of extensive studies of thiamin diphosphate, (ThDP) enzymes, there remain many uncertainties as to how these enzymes, achieve their rate enhancements. Here, we present a clear picture of, catalysis for the simple nonoxidative decarboxylase, oxalyl-coenzyme A, (CoA) decarboxylase, based on crystallographic snapshots along the, catalytic cycle and kinetic data on active site mutants. First, we provide, crystallographic evidence that, upon binding of oxalyl-CoA, the C-terminal, 13 residues fold over the substrate, aligning the substrate alpha-carbon, for attack by the ThDP-C2 atom. The second structure presented shows a, covalent reaction intermediate after decarboxylation, interpreted as being, nonplanar. Finally, the structure of a product complex is ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17637344 (full description)]]

==About this Structure==
2JI8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Oxalobacter_formigenes Oxalobacter formigenes]] with MG, TPP, ADP, FYN and PGE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Oxalyl-CoA_decarboxylase Oxalyl-CoA decarboxylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.8 4.1.1.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JI8 OCA]]. 

==Reference==
Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases., Berthold CL, Toyota CG, Moussatche P, Wood MD, Leeper F, Richards NG, Lindqvist Y, Structure. 2007 Jul;15(7):853-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17637344 17637344]
[[Category: Oxalobacter formigenes]]
[[Category: Oxalyl-CoA decarboxylase]]
[[Category: Single protein]]
[[Category: Berthold, C.L.]]
[[Category: Leeper, F.]]
[[Category: Lindqvist, Y.]]
[[Category: Moussatche, P.]]
[[Category: Richards, N.G.J.]]
[[Category: Toyota, C.G.]]
[[Category: Wood, M.D.]]
[[Category: ADP]]
[[Category: FYN]]
[[Category: MG]]
[[Category: PGE]]
[[Category: TPP]]
[[Category: decarboxylase]]
[[Category: flavoprotein]]
[[Category: lyase]]
[[Category: non- oxidative decarboxylase]]
[[Category: oxalate degradation]]
[[Category: product complex]]
[[Category: thiamin diphosphate-dependent]]
[[Category: thiamine pyrophosphate]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:48:13 2007''