View source for 2pzd

[[Image:2pzd.jpg|left|200px]]<br /><applet load="2pzd" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2pzd, resolution 2.750&Aring;" />
'''Crystal Structure of the HtrA2/Omi PDZ Domain Bound to a Phage-Derived Ligand (WTMFWV)'''<br />

==Overview==
The mitochondrial serine protease HtrA2/Omi helps to maintain, mitochondrial function by handling misfolded proteins in the intermembrane, space. In addition, HtrA2/Omi has been implicated as a proapoptotic factor, upon release into the cytoplasm during the cell death cascade. The protein, contains a C-terminal PDZ domain that packs against the protease active, site and inhibits proteolytic activity. Engagement of the PDZ domain by, peptide ligands has been shown to activate the protease and also has been, proposed to mediate substrate recognition. We report a detailed structural, and functional analysis of the human HtrA2/Omi PDZ domain using peptide, libraries and affinity assays to define specificity, X-ray crystallography, to view molecular details of PDZ-ligand interactions, and alanine-scanning, mutagenesis to probe the peptide-binding groove. We show that the, HtrA2/Omi PDZ domain recognizes both C-terminal and internal stretches of, extended, hydrophobic polypeptides. High-affinity ligand recognition, requires contacts with up to five hydrophobic side chains by distinct, sites on the PDZ domain. However, no particular residue type is absolutely, required at any position, and thus, the HtrA2/Omi PDZ domain appears to be, a promiscuous module adapted to recognize unstructured, hydrophobic, polypeptides. This type of specificity is consistent with the biological, role of HtrA2/Omi in mitochondria, which requires the recognition of, diverse, exposed stretches of hydrophobic sequences in misfolded proteins., The findings are less consistent with, but do not exclude, a role for the, PDZ domain in targeting the protease to specific substrates during, apoptosis.

==About this Structure==
2PZD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/HtrA2_peptidase HtrA2 peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.108 3.4.21.108] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZD OCA]. 

==Reference==
Structural and functional analysis of the ligand specificity of the HtrA2/Omi PDZ domain., Zhang Y, Appleton BA, Wu P, Wiesmann C, Sidhu SS, Protein Sci. 2007 Aug;16(8):1738-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17656586 17656586]
[[Category: Homo sapiens]]
[[Category: HtrA2 peptidase]]
[[Category: Single protein]]
[[Category: Appleton, B.A.]]
[[Category: Wiesmann, C.]]
[[Category: EDO]]
[[Category: apoptosis]]
[[Category: hydrolase]]
[[Category: mitochondria]]
[[Category: pdz domain]]
[[Category: peptide-binding module]]
[[Category: serine protease]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:17:55 2008''