View source for 2eww

[[Image:2eww.jpg|left|200px]]<br /><applet load="2eww" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2eww, resolution 3.20&Aring;" />
'''Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP'''<br />

==Overview==
PilT is a hexameric ATPase required for bacterial type IV pilus retraction, and surface motility. Crystal structures of ADP- and ATP-bound Aquifex, aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and, C-terminal RecA-like ATPase domains followed by a set of short C-terminal, helices. The hexamer is formed by extensive polar subunit interactions, between the ATPase core of one monomer and the N-terminal domain of the, next. An additional structure captures a nonsymmetric PilT hexamer in, which approach of invariant arginines from two subunits to the bound, nucleotide forms an enzymatically competent active site. A panel of pilT, mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We, present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this, hexameric motor. Our conclusions apply to the entire type II/IV secretion, ATPase family.

==About this Structure==
2EWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWW OCA]. 

==Reference==
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17355871 17355871]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Forest, K.T.]]
[[Category: Satyshur, K.A.]]
[[Category: ATP]]
[[Category: atpase]]
[[Category: hexameric pilt]]
[[Category: pilus retraction motor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:55:23 2008''