View source for 2jdf

[[Image:2jdf.jpg|left|200px]]<br /><applet load="2jdf" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2jdf, resolution 1.7&Aring;" />
'''HUMAN GAMMA-B CRYSTALLIN'''<br />

==Overview==
The concept of novel binding proteins as an alternative to antibodies has, undergone rapid development and is now ready for practical use in a wide, range of applications. Alternative binding proteins, based on suitable, scaffolds with desirable properties, are selected from combinatorial, libraries in vitro. Here, we describe an approach using a beta-sheet of, human gamma-B-crystallin to generate a universal binding site through, randomization of eight solvent-exposed amino acid residues selected, according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against, a variety of targets that differ considerably in size and structure. The, isolated Affilin variants can be produced in Escherichia coli as soluble, proteins and have a high level of thermodynamic stability. The crystal, structures of the human wild-type gamma-B-crystallin and a selected, Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human, gamma-B-crystallin tolerates amino acid exchanges with no major structural, change. We conclude that the intrinsically stable and easily expressed, gamma-B-crystallin provides a suitable framework for the generation of, novel binding molecules.

==About this Structure==
2JDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JDF OCA]. 

==Reference==
Affilin-Novel Binding Molecules Based on Human gamma-B-Crystallin, an All beta-Sheet Protein., Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U, J Mol Biol. 2007 Jun 22;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17628592 17628592]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Ebersbach, H.]]
[[Category: Fiedler, E.]]
[[Category: Fiedler, M.]]
[[Category: Fiedler, U.]]
[[Category: Proetzel, G.]]
[[Category: Reimann, C.]]
[[Category: Rudolph, R.]]
[[Category: Scheuermann, T.]]
[[Category: Stubbs, M.T.]]
[[Category: affilin]]
[[Category: artificial binding protein]]
[[Category: eye lens protein]]
[[Category: gamma crystallin]]
[[Category: oxidation]]
[[Category: phosphorylation]]
[[Category: polymorphism]]
[[Category: structural protein]]

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