View source for 2g79

[[Image:2g79.gif|left|200px]]<br /><applet load="2g79" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2g79, resolution 1.690&Aring;" />
'''Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution'''<br />

==Overview==
Rational redesign of the binding pocket of Cellular Retinoic Acid Binding, Protein II (CRABPII) has provided a mutant that can bind retinal as a, protonated Schiff base, mimicking the binding observed in rhodopsin. The, reengineering was accomplished through a series of choreographed, manipulations to ultimately orient the reactive species (the epsilon-amino, group of Lys132 and the carbonyl of retinal) in the proper geometry for, imine formation. The guiding principle was to achieve the appropriate, Burgi-Dunitz trajectory for the reaction to ensue. Through, crystallographic analysis of protein mutants incapable of forming the, requisite Schiff base, a highly ordered water molecule was identified as a, key culprit in orienting retinal in a nonconstructive manner. Removal of, the ordered water, along with placing reinforcing mutations to favor the, desired orientation of retinal, led to a triple mutant CRABPII protein, capable of nanomolar binding of retinal as a protonated Schiff base. The, high-resolution crystal structure of all-trans-retinal bound to the, CRABPII triple mutant (1.2 A resolution) unequivocally illustrates the, imine formed between retinal and the protein.

==About this Structure==
2G79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=RET:'>RET</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G79 OCA]. 

==Reference==
Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17447762 17447762]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Geiger, J.H.]]
[[Category: Vaezeslami, S.]]
[[Category: NA]]
[[Category: RET]]
[[Category: SO4]]
[[Category: beta barrel]]
[[Category: crabpii]]
[[Category: crystallography]]
[[Category: high resolution]]
[[Category: retinal]]
[[Category: retinoic acid]]
[[Category: retinoids]]
[[Category: x-ray]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:25 2008''