View source for 1olp

[[Image:1olp.gif|left|200px]]<br />
<applet load="1olp" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1olp, resolution 2.50&Aring;" />
'''ALPHA TOXIN FROM CLOSTRIDIUM ABSONUM'''<br />

==Overview==
Clostridium absonum phospholipase C (Caa) is a 42.7 kDa protein, which, shows 60% amino acid sequence identity with the Clostridium perfringens, phospholipase C, or alpha-toxin (Cpa), and has been isolated from patients, suffering from gas gangrene. We report the cloning and sequencing, purification, characterisation and crystal structure of the Caa enzyme., Caa had twice the phospholipid-hydrolysing (lecithinase) activity, 1.5, times the haemolytic activity and over seven times the activity towards, phosphatidylcholine-based liposomes when compared with Cpa. However, the, Caa enzyme had a lower activity than Cpa to the free (i.e. not in lipid, bilayer) substrate para-nitrophenylphosphorylcholine, towards, sphingomyelin-based liposomes and showed half the cytotoxicity. The lethal, dose ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14568535 (full description)]]

==About this Structure==
1OLP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_sardiniense Clostridium sardiniense]] with CA and ZN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OLP OCA]]. 

==Reference==
Clostridium absonum alpha-toxin: new insights into clostridial phospholipase C substrate binding and specificity., Clark GC, Briggs DC, Karasawa T, Wang X, Cole AR, Maegawa T, Jayasekera PN, Naylor CE, Miller J, Moss DS, Nakamura S, Basak AK, Titball RW, J Mol Biol. 2003 Oct 31;333(4):759-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14568535 14568535]
[[Category: Clostridium sardiniense]]
[[Category: Phospholipase C]]
[[Category: Single protein]]
[[Category: Basak, A.K.]]
[[Category: Briggs, D.C.]]
[[Category: CA]]
[[Category: ZN]]
[[Category: calcium binding]]
[[Category: gas gangrene determinant]]
[[Category: hydrolase]]
[[Category: membrane binding]]
[[Category: zinc phospholipase c]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:17:50 2007''