View source for 1olt

[[Image:1olt.gif|left|200px]]<br />
<applet load="1olt" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1olt, resolution 2.07&Aring;" />
'''COPROPORPHYRINOGEN III OXIDASE (HEMN) FROM ESCHERICHIA COLI IS A RADICAL SAM ENZYME.'''<br />

==Overview==
'Radical SAM' enzymes generate catalytic radicals by combining a 4Fe-4S, cluster and S-adenosylmethionine (SAM) in close proximity. We present the, first crystal structure of a Radical SAM enzyme, that of HemN, the, Escherichia coli oxygen-independent coproporphyrinogen III oxidase, at, 2.07 A resolution. HemN catalyzes the essential conversion of, coproporphyrinogen III to protoporphyrinogen IX during heme biosynthesis., HemN binds a 4Fe-4S cluster through three cysteine residues conserved in, all Radical SAM enzymes. A juxtaposed SAM coordinates the fourth Fe ion, through its amide nitrogen and carboxylate oxygen. The SAM sulfonium, sulfur is near both the Fe (3.5 A) and a neighboring sulfur of the cluster, (3.6 A), allowing single electron transfer from the 4Fe-4S cluster to the, SAM ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14633981 (full description)]]

==About this Structure==
1OLT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SAM and SF4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OLT OCA]]. 

==Reference==
Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes., Layer G, Moser J, Heinz DW, Jahn D, Schubert WD, EMBO J. 2003 Dec 1;22(23):6214-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14633981 14633981]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Heinz, D.W.]]
[[Category: Jahn, D.]]
[[Category: Layer, G.]]
[[Category: Moser, J.]]
[[Category: Schubert, W.D.]]
[[Category: SAM]]
[[Category: SF4]]
[[Category: 4fe-4s cluster]]
[[Category: decarboxylase]]
[[Category: heme biosynthesis]]
[[Category: oxidoreductase]]
[[Category: radical sam enzyme]]
[[Category: s-adenosyl-l-methionine]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:17:39 2007''