View source for 2jff

[[Image:2jff.jpg|left|200px]]<br /><applet load="2jff" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2jff, resolution 1.89&Aring;" />
'''CRYSTAL STRUCTURE OF MURD LIGASE IN COMPLEX WITH D-GLU CONTAINING SULFONAMIDE INHIBITOR'''<br />

==Overview==
Mur ligases play an essential role in the intracellular biosynthesis of, bacterial peptidoglycan, the main component of the bacterial cell wall, and represent attractive targets for the design of novel antibacterials., UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) catalyses the, addition of D-glutamic acid to the cytoplasmic intermediate, UDP-N-acetylmuramoyl-L-alanine (UMA) and is the second in the series of, Mur ligases. MurD ligase is highly stereospecific for its substrate, D-glutamic acid (D-Glu). Here, we report the high resolution crystal, structures of MurD in complexes with two novel inhibitors designed to, mimic the transition state of the reaction, which contain either the D-Glu, or the L-Glu moiety. The binding modes of N-sulfonyl-D-Glu and, N-sulfonyl-L-Glu derivatives were also characterised kinetically. The, results of this study represent an excellent starting point for further, development of novel inhibitors of this enzyme.

==About this Structure==
2JFF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 and LK2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/UDP-N-acetylmuramoyl-L-alanine--D-glutamate_ligase UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.9 6.3.2.9] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JFF OCA]. 

==Reference==
Structural and functional characterization of enantiomeric glutamic acid derivatives as potential transition state analogue inhibitors of MurD ligase., Kotnik M, Humljan J, Contreras-Martel C, Oblak M, Kristan K, Herve M, Blanot D, Urleb U, Gobec S, Dessen A, Solmajer T, J Mol Biol. 2007 Jun 29;370(1):107-15. Epub 2007 May 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17507028 17507028]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase]]
[[Category: Blanot, D.]]
[[Category: Contreras-Martel, C.]]
[[Category: Dessen, A.]]
[[Category: Gobec, S.]]
[[Category: Herve, M.]]
[[Category: Humljan, J.]]
[[Category: Kotnik, M.]]
[[Category: Kristan, K.]]
[[Category: Oblak, M.]]
[[Category: Solmajer, T.]]
[[Category: Urleb, U.]]
[[Category: LK2]]
[[Category: SO4]]
[[Category: atp-binding]]
[[Category: cell cycle]]
[[Category: cell division]]
[[Category: cell shape]]
[[Category: cell wall]]
[[Category: ligase]]
[[Category: murd ligase]]
[[Category: murd-inhibitor complex]]
[[Category: nucleotide-binding]]
[[Category: peptidoglycan synthesis]]
[[Category: sulfonamide inhibitor]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:05:53 2007''