View source for 2j9p

[[Image:2j9p.jpg|left|200px]]<br /><applet load="2j9p" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2j9p, resolution 2.80&Aring;" />
'''CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS PBP4A, AND ITS COMPLEX WITH A PEPTIDOGLYCAN MIMETIC PEPTIDE.'''<br />

==Overview==
The genome of Bacillus subtilis encodes 16 penicillin-binding proteins, (PBPs) involved in the synthesis and/or remodelling of the peptidoglycan, during the complex life cycle of this sporulating Gram-positive rod-shaped, bacterium. PBP4a (encoded by the dacC gene) is a low-molecular mass PBP, clearly exhibiting in vitrodd-carboxypeptidase activity. We have solved, the crystal structure of this protein alone and in complex with a peptide, (d-alpha-aminopymelyl-epsilon-d-alanyl-d-alanine) that mimics the, C-terminal end of the Bacillus peptidoglycan stem peptide. PBP4a is, composed of three domains: the penicillin-binding domain with a fold, similar to the class A beta-lactamase structure and two domains inserted, between the conserved motifs 1 and 2 characteristic of the, penicillin-recognizing enzymes. The soaking of PBP4a in a solution of, d-alpha-aminopymelyl-epsilon-d-alanyl-d-alanine resulted in an adduct, between PBP4a and a d-alpha-aminopimelyl-epsilon-d-alanine dipeptide and, an unbound d-alanine, i.e. the products of acylation of PBP4a by, d-alpha-aminopymelyl-epsilon-d-alanyl-d-alanine with the release of a, d-alanine. The adduct also reveals a binding pocket specific to the, diaminopimelic acid, the third residue of the peptidoglycan stem, pentapeptide of B. subtilis. This pocket is specific for this class of, PBPs.

==About this Structure==
2J9P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with REZ as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Dal Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J9P OCA]. 

==Reference==
Crystal Structure of the Bacillus subtilis Penicillin-binding Protein 4a, and its Complex with a Peptidoglycan Mimetic Peptide., Sauvage E, Duez C, Herman R, Kerff F, Petrella S, Anderson JW, Adediran SA, Pratt RF, Frere JM, Charlier P, J Mol Biol. 2007 Aug 10;371(2):528-39. Epub 2007 Jun 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17582436 17582436]
[[Category: Single protein]]
[[Category: Charlier, P.]]
[[Category: Duez, C.]]
[[Category: Herman, R.]]
[[Category: Kerff, F.]]
[[Category: Sauvage, E.]]
[[Category: REZ]]
[[Category: bacillus subtilis]]
[[Category: cell cycle]]
[[Category: cell division]]
[[Category: cell shape]]
[[Category: cell wall]]
[[Category: d-ala-d-ala- carboxypeptidase]]
[[Category: hydrolase]]
[[Category: penicillin-binding protein]]
[[Category: peptidoglycan]]
[[Category: peptidoglycan synthesis]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:56:42 2007''