View source for 2cci

[[Image:2cci.gif|left|200px]]<br />
<applet load="2cci" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2cci, resolution 2.70&Aring;" />
'''CRYSTAL STRUCTURE OF PHOSPHO-CDK2 CYCLIN A IN COMPLEX WITH A PEPTIDE CONTAINING BOTH THE SUBSTRATE AND RECRUITMENT SITES OF CDC6'''<br />

==Overview==
Phospho-CDK2/cyclin A, a kinase that is active in cell cycle S phase, contains an RXL substrate recognition site that is over 40 A from the, catalytic site. The role of this recruitment site, which enhances, substrate affinity and catalytic efficiency, has been investigated using, peptides derived from the natural substrates, namely CDC6 and p107, and a, bispeptide inhibitor in which the gamma-phosphate of ATP is covalently, attached by a linker to the CDC6 substrate peptide. X-ray studies with a, 30-residue CDC6 peptide in complex with pCDK2/cyclin A showed binding of a, dodecamer peptide at the recruitment site and a heptapeptide at the, catalytic site, but no density for the linking 11 residues. Kinetic, studies established that the CDC6 peptide had an 18-fold lower Km compared, with ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16707497 (full description)]]

==About this Structure==
2CCI is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and ATP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_2.7.11.1 Transferred entry: 2.7.11.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CCI OCA]]. 

==Reference==
The role of the phospho-CDK2/cyclin A recruitment site in substrate recognition., Cheng KY, Noble ME, Skamnaki V, Brown NR, Lowe ED, Kontogiannis L, Shen K, Cole PA, Siligardi G, Johnson LN, J Biol Chem. 2006 Aug 11;281(32):23167-79. Epub 2006 May 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16707497 16707497]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Transferred entry: 2.7.11.1]]
[[Category: Brown, N.R.]]
[[Category: Cheng, K.Y.]]
[[Category: Cole, P.A.]]
[[Category: Johnson, L.N.]]
[[Category: Kontogiannis, L.]]
[[Category: Lowe, E.D.]]
[[Category: Noble, M.E.M.]]
[[Category: Shen, K.]]
[[Category: Siligardi, G.]]
[[Category: Skamnaki, V.]]
[[Category: ATP]]
[[Category: MG]]
[[Category: atp-binding]]
[[Category: cell cycle]]
[[Category: cell division]]
[[Category: complex]]
[[Category: cyclin]]
[[Category: dna replication]]
[[Category: kinase]]
[[Category: mitosis]]
[[Category: nuclear protein]]
[[Category: nucleotide-binding]]
[[Category: phosphorylation]]
[[Category: protein kinases]]
[[Category: recruitment]]
[[Category: serine/threonine-protein kinase]]
[[Category: substrate recognition]]
[[Category: transferase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:50:32 2007''