View source for 1qlg

[[Image:1qlg.jpg|left|200px]]<br /><applet load="1qlg" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1qlg, resolution 2.2&Aring;" />
'''CRYSTAL STRUCTURE OF PHYTASE WITH MAGNESIUM FROM BACILLUS AMYLOLIQUEFACIENS'''<br />

==Overview==
Phytases hydrolyze phytic acid to less phosphorylated myo-inositol, derivatives and inorganic phosphate. A thermostable phytase is of great, value in applications for improving phosphate and metal ion availability, in animal feed, and thereby reducing phosphate pollution to the, environment. Here, we report a new folding architecture of a six-bladed, propeller for phosphatase activity revealed by the 2.1 A crystal, structures of a novel, thermostable phytase determined in both the, partially and fully Ca2+-loaded states. Binding of two calcium ions to, high-affinity calcium binding sites results in a dramatic increase in, thermostability (by as much as approximately 30 degrees C in melting, temperature) by joining loop segments remote in the amino acid sequence., Binding of three additional calcium ions to low-affinity calcium binding, sites at the top of the molecule turns on the catalytic activity of the, enzyme by converting the highly negatively charged cleft into a favorable, environment for the binding of phytate.

==About this Structure==
1QLG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] with CA and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Known structural/functional Site: <scene name='pdbsite=MG:Mg'>MG</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QLG OCA]. 

==Reference==
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states., Ha NC, Oh BC, Shin S, Kim HJ, Oh TK, Kim YO, Choi KY, Oh BH, Nat Struct Biol. 2000 Feb;7(2):147-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10655618 10655618]
[[Category: 3-phytase]]
[[Category: Bacillus amyloliquefaciens]]
[[Category: Single protein]]
[[Category: Ha, N.C.]]
[[Category: Oh, B.H.]]
[[Category: Shin, S.]]
[[Category: CA]]
[[Category: MG]]
[[Category: calcium]]
[[Category: magnesium]]
[[Category: phosphatase]]
[[Category: phytase]]
[[Category: thermostable]]

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