View source for 1e42

[[Image:1e42.gif|left|200px]]<br />
<applet load="1e42" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1e42, resolution 1.70&Aring;" />
'''BETA2-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2'''<br />

==Overview==
The heterotetrameric AP2 adaptor (alpha, beta 2, mu 2 and sigma 2, subunits) plays a central role in clathrin-mediated endocytosis. We, present the protein recruitment function and 1.7 A resolution structure of, its beta 2-appendage domain to complement those previously determined for, the mu 2 subunit and alpha appendage. Using structure-directed, mutagenesis, we demonstrate the ability of the beta 2 appendage alone to, bind directly to clathrin and the accessory proteins AP180, epsin and, eps15 at the same site. Clathrin polymerization is promoted by binding of, clathrin simultaneously to the beta 2-appendage site and to a second site, on the adjacent beta 2 hinge. This results in the displacement of the, other ligands from the beta 2 appendage. Thus clathrin binding to an, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10944104 (full description)]]

==About this Structure==
1E42 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CL, MG, NI, DTD and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: AC1, AC2, AC3, AC4 and AC5. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E42 OCA]]. 

==Reference==
The structure and function of the beta 2-adaptin appendage domain., Owen DJ, Vallis Y, Pearse BM, McMahon HT, Evans PR, EMBO J. 2000 Aug 15;19(16):4216-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10944104 10944104]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Evans, P.R.]]
[[Category: Mcmahon, H.T.]]
[[Category: Owen, D.J.]]
[[Category: CL]]
[[Category: DTD]]
[[Category: GOL]]
[[Category: MG]]
[[Category: NI]]
[[Category: adaptor]]
[[Category: endocytosis]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:05:01 2007''