View source for 1cvx

[[Image:1cvx.gif|left|200px]]<br /><applet load="1cvx" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1cvx, resolution 2.27&Aring;" />
'''CRYSTAL STRUCTURE OF POLYAMIDE DIMER (IMPYHPPYBETADP)2 BOUND TO B-DNA DECAMER CCAGATCTGG'''<br />

==Overview==
Synthetic polyamides composed of three types of aromatic amino acids, N-methylimidazole (Im), N-methylpyrrole (Py) and N-methyl-3-hydroxypyrrole, (Hp) bind specific DNA sequences as antiparallel dimers in the minor, groove. The side-by-side pairings of aromatic rings in the dimer afford a, general recognition code that allows all four base-pairs to be, distinguished. To examine the structural consequences of changing the DNA, sequence context on T.A recognition by Hp/Py pairs in the minor groove, crystal structures of polyamide dimers (ImPyHpPy)(2) and the pyrrole, counterpart (ImPyPyPy)(2) bound to the six base-pair target site, 5'-AGATCT-3' in a ten base-pair oligonucleotide have been determined to a, resolution of 2.27 and 2.15 A, respectively. The structures demonstrate, that the principles of Hp/Py recognition of T.A are consistent between, different sequence contexts. However, a general structural explanation for, the non-additive reduction in binding affinity due to introduction of the, hydroxyl group is less clear. Comparison with other polyamide-DNA, cocrystal structures reveals structural themes and differences that may, relate to sequence preference.

==About this Structure==
1CVX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with HP2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CVX OCA]. 

==Reference==
Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove., Kielkopf CL, Bremer RE, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, J Mol Biol. 2000 Jan 21;295(3):557-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10623546 10623546]
[[Category: Protein complex]]
[[Category: Baird, E.E.]]
[[Category: Bremer, R.E.]]
[[Category: Dervan, P.B.]]
[[Category: Kielkopf, C.L.]]
[[Category: Rees, D.C.]]
[[Category: White, S.]]
[[Category: HP2]]
[[Category: double drug in minor groove]]
[[Category: minor groove recognition]]
[[Category: polyamide]]
[[Category: ta recognition]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:00:47 2007''