View source for 1xbn

[[Image:1xbn.gif|left|200px]]<br /><applet load="1xbn" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1xbn, resolution 2.50&Aring;" />
'''Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain'''<br />

==Overview==
Nitric oxide (NO) is extremely toxic to Clostridium botulinum, but its, molecular targets are unknown. Here, we identify a heme protein sensor, (SONO) that displays femtomolar affinity for NO. The crystal structure of, the SONO heme domain reveals a previously undescribed fold and a, strategically placed tyrosine residue that modulates heme-nitrosyl, coordination. Furthermore, the domain architecture of a SONO ortholog, cloned from Chlamydomonas reinhardtii indicates that NO signaling through, cyclic guanosine monophosphate arose before the origin of multicellular, eukaryotes. Our findings have broad implications for understanding, bacterial responses to NO, as well as for the activation of mammalian, NO-sensitive guanylyl cyclase.

==About this Structure==
1XBN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with OXY and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XBN OCA]. 

==Reference==
Femtomolar sensitivity of a NO sensor from Clostridium botulinum., Nioche P, Berka V, Vipond J, Minton N, Tsai AL, Raman CS, Science. 2004 Nov 26;306(5701):1550-3. Epub 2004 Oct 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15472039 15472039]
[[Category: Single protein]]
[[Category: Thermoanaerobacter tengcongensis]]
[[Category: Nioche, P.]]
[[Category: Raman, C.S.]]
[[Category: HEM]]
[[Category: OXY]]
[[Category: cgmp]]
[[Category: heme protein]]
[[Category: nitric oxide]]
[[Category: soluble guanylyl cyclase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:48:49 2007''