View source for 8vrs


==Mucin 16 peptide fused to MBP in complex with 4H11-scFv antibody==
<StructureSection load='8vrs' size='340' side='right'caption='[[8vrs]], [[Resolution|resolution]] 2.47&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8vrs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VRS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vrs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vrs OCA], [https://pdbe.org/8vrs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vrs RCSB], [https://www.ebi.ac.uk/pdbsum/8vrs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vrs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/MUC16_HUMAN MUC16_HUMAN] Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Mucin 16 (MUC16) overexpression is linked with cancer progression, metastasis, and therapy resistance in high grade serous ovarian cancer and other malignancies. The cleavage of MUC16 forms independent bimodular fragments, the shed tandem repeat sequence which circulates as a protein bearing the ovarian cancer biomarker (CA125) and a proximal membrane-bound component which is critical in MUC16 oncogenic behavior. A humanized, high affinity antibody targeting the proximal ectodomain represents a potential therapeutic agent against MUC16 with lower antigenic potential and restricted human tissue expression. RESULTS: Here, we demonstrate the potential therapeutic versatility of the humanized antibody as a monoclonal antibody, antibody drug conjugate, and chimeric antigen receptor. We report the crystal structures of 4H11-scFv, derived from an antibody specifically targeting the MUC16 C-terminal region, alone and in complex with a 26-amino acid MUC16 segment resolved at 2.36 A and 2.47 A resolution, respectively. The scFv forms a robust interaction with an epitope consisting of two consecutive beta-turns and a beta-hairpin stabilized by 2 hydrogen bonds. The V(H)-V(L) interface within the 4H11-scFv is stabilized through an intricate network of 11 hydrogen bonds and a cation-pi interaction. CONCLUSIONS: Together, our studies offer insight into antibody-MUC16 ectodomain interaction and advance our ability to design agents with potentially improved therapeutic properties over anti-CA125 moiety antibodies.

Structural basis for antibody recognition of the proximal MUC16 ectodomain.,Lee K, Perry K, Xu M, Veillard I, Kumar R, Rao TD, Rueda BR, Spriggs DR, Yeku OO J Ovarian Res. 2024 Feb 19;17(1):41. doi: 10.1186/s13048-024-01373-9. PMID:38374055<ref>PMID:38374055</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8vrs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Lee K]]
[[Category: Perry K]]
[[Category: Spriggs DR]]
[[Category: Yeku OO]]