View source for 7msu


==Crystal structure of an archaeal CNNM, MtCorB, CBS-pair domain in complex with Mg2+-ATP==
<StructureSection load='7msu' size='340' side='right'caption='[[7msu]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7msu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanoculleus_thermophilus Methanoculleus thermophilus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7lj6 7lj6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7msu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msu OCA], [https://pdbe.org/7msu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7msu RCSB], [https://www.ebi.ac.uk/pdbsum/7msu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7msu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1G8XA46_9EURY A0A1G8XA46_9EURY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CNNM/CorB proteins are a broadly conserved family of integral membrane proteins with close to 90,000 protein sequences known. They are associated with Mg(2+) transport but it is not known if they mediate transport themselves or regulate other transporters. Here, we determine the crystal structure of an archaeal CorB protein in two conformations (apo and Mg(2+)-ATP bound). The transmembrane DUF21 domain exists in an inward-facing conformation with a Mg(2+) ion coordinated by a conserved pi-helix. In the absence of Mg(2+)-ATP, the CBS-pair domain adopts an elongated dimeric configuration with previously unobserved domain-domain contacts. Hydrogen-deuterium exchange mass spectrometry, analytical ultracentrifugation, and molecular dynamics experiments support a role of the structural rearrangements in mediating Mg(2+)-ATP sensing. Lastly, we use an in vitro, liposome-based assay to demonstrate direct Mg(2+) transport by CorB proteins. These structural and functional insights provide a framework for understanding function of CNNMs in Mg(2+) transport and associated diseases.

Crystal structure of an archaeal CorB magnesium transporter.,Chen YS, Kozlov G, Moeller BE, Rohaim A, Fakih R, Roux B, Burke JE, Gehring K Nat Commun. 2021 Jun 29;12(1):4028. doi: 10.1038/s41467-021-24282-7. PMID:34188059<ref>PMID:34188059</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7msu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Hemolysin 3D structures|Hemolysin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanoculleus thermophilus]]
[[Category: Chen YS]]
[[Category: Gehring K]]