View source for 6ril


==Single crystal serial study of the inhibition of laccases from Steccherinum murashkinskyi by fluoride anions at sub-atomic resolution. Fourteenth structure of the series with 5600 KGy dose (data was collected after refreezing).==
<StructureSection load='6ril' size='340' side='right'caption='[[6ril]], [[Resolution|resolution]] 1.34&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ril]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Steccherinum_murashkinskyi Steccherinum murashkinskyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RIL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.34&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ril FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ril OCA], [https://pdbe.org/6ril PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ril RCSB], [https://www.ebi.ac.uk/pdbsum/6ril PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ril ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I1VE66_9APHY I1VE66_9APHY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Laccases are enzymes that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of molecular oxygen to water. Here, a subatomic resolution X-ray crystallographic study of the mechanism of inhibition of the laccase from the basidiomycete fungus Steccherinum murashkinskyi by chloride and fluoride ions is presented. Three series of X-ray diffraction data sets were collected with increasing doses of absorbed X-ray radiation from a native S. murashkinskyi laccase crystal and from crystals of complexes of the laccase with chloride and fluoride ions. The data for the native laccase crystal confirmed the previously deduced enzymatic mechanism of molecular oxygen reduction. The structures of the complexes allowed the localization of chloride and fluoride ions in the channel near the T2 copper ion. These ions replace the oxygen ligand of the T2 copper ion in this channel and can play the role of this ligand in the enzymatic reaction. As follows from analysis of the structures from the increasing dose series, the inhibition of laccases by chloride and fluoride anions can be explained by the fact that the binding of these negatively charged ions at the position of the oxygen ligand of the T2 copper ion impedes the reduction of the T2 copper ion.

The subatomic resolution study of laccase inhibition by chloride and fluoride anions using single-crystal serial crystallography: insights into the enzymatic reaction mechanism.,Polyakov KM, Gavryushov S, Fedorova TV, Glazunova OA, Popov AN Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):804-816. doi: , 10.1107/S2059798319010684. Epub 2019 Aug 22. PMID:31478903<ref>PMID:31478903</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ril" style="background-color:#fffaf0;"></div>

==See Also==
*[[Laccase 3D structures|Laccase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Steccherinum murashkinskyi]]
[[Category: Fedorova TV]]
[[Category: Gavryushov S]]
[[Category: Glazunova OA]]
[[Category: Polyakov KM]]
[[Category: Popov AN]]