View source for 6pkm


==MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8)==
<StructureSection load='6pkm' size='340' side='right'caption='[[6pkm]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6pkm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PKM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pkm OCA], [https://pdbe.org/6pkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pkm RCSB], [https://www.ebi.ac.uk/pdbsum/6pkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pkm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues.
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== Publication Abstract from PubMed ==
Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.

Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.,Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T Structure. 2019 Oct 1;27(10):1594-1600.e2. doi: 10.1016/j.str.2019.07.004. Epub, 2019 Aug 15. PMID:31422911<ref>PMID:31422911</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6pkm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Proteinase 3D structures|Proteinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Parengyodontium album]]
[[Category: Gonen T]]
[[Category: Hattne J]]
[[Category: Jensen GJ]]
[[Category: Martynowycz MW]]
[[Category: Zhao W]]