View source for 6j43


==Proteinase K determined by PAL-XFEL==
<StructureSection load='6j43' size='340' side='right'caption='[[6j43]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J43 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j43 OCA], [https://pdbe.org/6j43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j43 RCSB], [https://www.ebi.ac.uk/pdbsum/6j43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j43 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 A resolution.

Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.,Lee D, Park S, Lee K, Kim J, Park G, Nam KH, Baek S, Chung WK, Lee JL, Cho Y, Park J J Appl Crystallogr. 2020 Mar 25;53(Pt 2):477-485. doi: 10.1107/S1600576720002423., eCollection 2020 Apr 1. PMID:32280322<ref>PMID:32280322</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6j43" style="background-color:#fffaf0;"></div>

==See Also==
*[[Proteinase 3D structures|Proteinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lee SJ]]
[[Category: Park J]]