View source for 6izv


==Averaged strand structure of a 15-stranded ParM filament from Clostridium botulinum==
<SX load='6izv' size='340' side='right' viewer='molstar' caption='[[6izv]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6izv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_Prevot_594 Clostridium botulinum Prevot_594]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IZV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6izv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izv OCA], [https://pdbe.org/6izv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6izv RCSB], [https://www.ebi.ac.uk/pdbsum/6izv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6izv ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.

The structure of a 15-stranded actin-like filament from Clostridium botulinum.,Koh F, Narita A, Lee LJ, Tanaka K, Tan YZ, Dandey VP, Popp D, Robinson RC Nat Commun. 2019 Jun 28;10(1):2856. doi: 10.1038/s41467-019-10779-9. PMID:31253774<ref>PMID:31253774</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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[[Category: Clostridium botulinum Prevot_594]]
[[Category: Large Structures]]
[[Category: Dandey VP]]
[[Category: Koh F]]
[[Category: Lee LJ]]
[[Category: Narita A]]
[[Category: Popp D]]
[[Category: Robinson RC]]
[[Category: Tan YZ]]
[[Category: Tanaka K]]