View source for 6gz4


==tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)==
<SX load='6gz4' size='340' side='right' viewer='molstar' caption='[[6gz4]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gz4]] is a 86 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GZ4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6gz3|6gz3]], [[6gz5|6gz5]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gz4 OCA], [https://pdbe.org/6gz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gz4 RCSB], [https://www.ebi.ac.uk/pdbsum/6gz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gz4 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Translocation moves the tRNA2mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA2mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site.

tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.,Flis J, Holm M, Rundlet EJ, Loerke J, Hilal T, Dabrowski M, Burger J, Mielke T, Blanchard SC, Spahn CMT, Budkevich TV Cell Rep. 2018 Dec 4;25(10):2676-2688.e7. doi: 10.1016/j.celrep.2018.11.040. PMID:30517857<ref>PMID:30517857</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6gz4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Blanchard, S C]]
[[Category: Budkevich, T V]]
[[Category: Buerger, J]]
[[Category: Dabrowski, M]]
[[Category: Flis, J]]
[[Category: Hilal, T]]
[[Category: Holm, M]]
[[Category: Loerke, J]]
[[Category: Mielke, T]]
[[Category: Rundlet, E J]]
[[Category: Spahn, C M.T]]
[[Category: Dde]]
[[Category: Diphthamide]]
[[Category: Eef2]]
[[Category: Ef-g]]
[[Category: Gmppnp]]
[[Category: Mammalian]]
[[Category: Ribosome]]
[[Category: Translocation]]