View source for 6ere


==Crystal structure of a computationally designed colicin endonuclease and immunity pair colEdes3/Imdes3==
<StructureSection load='6ere' size='340' side='right'caption='[[6ere]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ere]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ERE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ERE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6er6|6er6]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ere FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ere OCA], [http://pdbe.org/6ere PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ere RCSB], [http://www.ebi.ac.uk/pdbsum/6ere PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ere ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Protein networks in all organisms comprise homologous interacting pairs. In these networks, some proteins are specific, interacting with one or a few binding partners, whereas others are multispecific and bind a range of targets. We describe an algorithm that starts from an interacting pair and designs dozens of new pairs with diverse backbone conformations at the binding site as well as new binding orientations and sequences. Applied to a high-affinity bacterial pair, the algorithm results in 18 new ones, with cognate affinities from pico- to micromolar. Three pairs exhibit 3-5 orders of magnitude switch in specificity relative to the wild type, whereas others are multispecific, collectively forming a protein-interaction network. Crystallographic analysis confirms design accuracy, including in new backbones and polar interactions. Preorganized polar interaction networks are responsible for high specificity, thus defining design principles that can be applied to program synthetic cellular interaction networks of desired affinity and specificity.

Ultrahigh specificity in a network of computationally designed protein-interaction pairs.,Netzer R, Listov D, Lipsh R, Dym O, Albeck S, Knop O, Kleanthous C, Fleishman SJ Nat Commun. 2018 Dec 11;9(1):5286. doi: 10.1038/s41467-018-07722-9. PMID:30538236<ref>PMID:30538236</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ere" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Large Structures]]
[[Category: Albeck, S]]
[[Category: Dym, O]]
[[Category: Fleishman, S J]]
[[Category: Knop, O]]
[[Category: Listov, D]]
[[Category: Netzer, R]]
[[Category: Immune system]]
[[Category: Immunity colicin]]