View source for 6abv


==Crystal structure of citrate synthase (Msed_0281) from Metallosphaera sedula==
<StructureSection load='6abv' size='340' side='right'caption='[[6abv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6abv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_sedula_DSM_5348 Metallosphaera sedula DSM 5348]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ABV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6abv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6abv OCA], [https://pdbe.org/6abv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6abv RCSB], [https://www.ebi.ac.uk/pdbsum/6abv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6abv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4YDF6_METS5 A4YDF6_METS5] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metallosphaera sedula is a thermoacidophilic archaeon that has carbon fixation ability using the 3-hydroxypropionate/4-hydroxybutyrate(3-HP/4-HB) cycle, and has an incomplete TCA cycle to produce necessary biosynthetic precursors. The citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA cycle, catalyzing the conversion of oxaloacetate and acetyl-CoA into citrate and coenzyme A. To investigate the molecular mechanism of MsCS, we determined its crystal structure at 1.8A resolution. As other known CSs, MsCS functions as a dimer, and each monomer consists of two domains, a large domain and a small domain. We also determined the structure of the complex with acetyl-CoA and revealed the acetyl-CoA binding mode of MsCS. Structural comparison of MsCS with another CS in complex with oxaloacetate enabled us to predict the oxaloacetate binding site. Moreover, we performed inhibitory kinetic analyses of MsCS, and showed that the protein is inhibited by citrate and ATP by competitive and non-competitive inhibition modes, respectively, but not by NADH. Based on these results, we suggest that MsCS belongs to the type-I CS with structural and biochemical properties similar to those of CSs involved in the conventional TCA cycle.

Crystal structure and biochemical properties of msed_0281, the citrate synthase from Metallosphaera sedula.,Lee SH, Kim KJ Biochem Biophys Res Commun. 2019 Jan 2. pii: S0006-291X(18)32860-2. doi:, 10.1016/j.bbrc.2018.12.172. PMID:30611567<ref>PMID:30611567</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6abv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Citrate Synthase 3D structures|Citrate Synthase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Metallosphaera sedula DSM 5348]]
[[Category: Kim K-J]]
[[Category: Lee S-H]]