View source for 5wc4


==Crystal structure of biphenyl synthase from Malus domestic complexed with benzoyl-CoA==
<StructureSection load='5wc4' size='340' side='right'caption='[[5wc4]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wc4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WC4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WC4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU4:(3R)-BUTANE-1,3-DIOL'>BU4</scene>, <scene name='pdbligand=BYC:BENZOYL+COENZYME+A'>BYC</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w8q|5w8q]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3,5-dihydroxybiphenyl_synthase 3,5-dihydroxybiphenyl synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.177 2.3.1.177] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wc4 OCA], [http://pdbe.org/5wc4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wc4 RCSB], [http://www.ebi.ac.uk/pdbsum/5wc4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wc4 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Biphenyl synthase and benzophenone synthase constitute an evolutionarily distinct clade of type III polyketide synthases (PKSs) that use benzoic acid-derived substrates to produce defense metabolites in plants. The use of benzoyl-CoA as an endogenous substrate is unusual for type III PKSs. Moreover, sequence analyses indicate that the residues responsible for the functional diversification of type III PKSs are mutated in benzoic acid-specific type III PKSs. In order to gain a better understanding of structure-function relationships within the type III PKS family, the crystal structures of biphenyl synthase from Malus x domestica and benzophenone synthase from Hypericum androsaemum were compared with the structure of an archetypal type III PKS: chalcone synthase from Malus x domestica. Both biphenyl synthase and benzophenone synthase contain mutations that reshape their active-site cavities to prevent the binding of 4-coumaroyl-CoA and to favor the binding of small hydrophobic substrates. The active-site cavities of biphenyl synthase and benzophenone synthase also contain a novel pocket associated with their chain-elongation and cyclization reactions. Collectively, these results illuminate structural determinants of benzoic acid-specific type III PKSs and expand the understanding of the evolution of specialized metabolic pathways in plants.

Molecular architectures of benzoic acid-specific type III polyketide synthases.,Stewart C Jr, Woods K, Macias G, Allan AC, Hellens RP, Noel JP Acta Crystallogr D Struct Biol. 2017 Dec 1;73(Pt 12):1007-1019. doi:, 10.1107/S2059798317016618. Epub 2017 Nov 30. PMID:29199980<ref>PMID:29199980</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: 3,5-dihydroxybiphenyl synthase]]
[[Category: Large Structures]]
[[Category: Jr, C E.Stewart]]
[[Category: Noel, J P]]
[[Category: Polyketide synthase]]
[[Category: Thiolase fold]]
[[Category: Transferase]]