View source for 5v8d


==Structure of Bacillus cereus PatB1 with sulfonyl adduct==
<StructureSection load='5v8d' size='340' side='right'caption='[[5v8d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5v8d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_10987 Bacillus cereus ATCC 10987]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V8D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OSE:O-SULFO-L-SERINE'>OSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8d OCA], [https://pdbe.org/5v8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v8d RCSB], [https://www.ebi.ac.uk/pdbsum/5v8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q73CU0_BACC1 Q73CU0_BACC1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.

PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.,Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ Nat Chem Biol. 2017 Oct 30. pii: nchembio.2509. doi: 10.1038/nchembio.2509. PMID:29083419<ref>PMID:29083419</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5v8d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus cereus ATCC 10987]]
[[Category: Large Structures]]
[[Category: Boons GJ]]
[[Category: Chapman RN]]
[[Category: Clarke AJ]]
[[Category: Howell PL]]
[[Category: Little DJ]]
[[Category: Robinson H]]
[[Category: Sychantha D]]