View source for 5o2w


==Extended catalytic domain of Hypocrea jecorina LPMO 9A.==
<StructureSection load='5o2w' size='340' side='right'caption='[[5o2w]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5o2w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei_QM6a Trichoderma reesei QM6a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O2W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o2w OCA], [https://pdbe.org/5o2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o2w RCSB], [https://www.ebi.ac.uk/pdbsum/5o2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o2w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0R6T8_HYPJQ G0R6T8_HYPJQ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
For decades, the enzymes of the fungus Hypocrea jecorina have served as a model system for the breakdown of cellulose. Three-dimensional structures for almost all H. jecorina cellulose-degrading enzymes are available, except for HjLPMO9A, belonging to the AA9 family of lytic polysaccharide monooxygenases (LPMOs). These enzymes enhance the hydrolytic activity of cellulases and are essential for cost-efficient conversion of lignocellulosic biomass. Here, using structural and spectroscopic analyses, we found that native HjLPMO9A contains a catalytic domain and a family-1 carbohydrate-binding module (CBM1) connected via a linker sequence. A C-terminally truncated variant of HjLPMO9A containing 21 residues of the predicted linker expressed at levels sufficient for analysis. Here, using structural, spectroscopic and biochemical analyses, we found that this truncated variant exhibited reduced binding to and activity on cellulose compared with the full-length enzyme. Importantly, a 0.95 A resolution X-ray structure of truncated HjLPMO9A revealed that the linker forms an integral part of the catalytic domain structure, covering a hydrophobic patch on the catalytic AA9 module. We noted that the oxidized catalytic center contains a Cu(II) coordinated by two His ligands, one of which has a Hisbrace in which the His1 terminal amine group also coordinates to a copper. The final equatorial position of the Cu(II) is occupied by a water-derived ligand. The spectroscopic characteristics of the truncated variant were not measurably different from those of full-length HjLPMO9A, indicating that the presence of the CBM1 module increases the affinity of HjLPMO9A for cellulose binding, but does not affect the active site.

High-resolution structure of a lytic polysaccharide monooxygenase from Hypocrea jecorina reveals a predicted linker as an integral part of the catalytic domain.,Hansson H, Karkehabadi S, Mikkelsen N, Douglas NR, Kim S, Lam A, Kaper T, Kelemen B, Meier KK, Jones SM, Solomon EI, Sandgren M J Biol Chem. 2017 Sep 12. pii: jbc.M117.799767. doi: 10.1074/jbc.M117.799767. PMID:28900033<ref>PMID:28900033</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5o2w" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trichoderma reesei QM6a]]
[[Category: Hansson H]]
[[Category: Karkehabadi S]]
[[Category: Mikelssen NE]]
[[Category: Sandgren M]]