View source for 5nq9


==Crystal structure of laccases from Pycnoporus sanguineus, izoform II, monoclinic==
<StructureSection load='5nq9' size='340' side='right'caption='[[5nq9]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nq9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trametes_sanguinea Trametes sanguinea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NQ9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq9 OCA], [https://pdbe.org/5nq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nq9 RCSB], [https://www.ebi.ac.uk/pdbsum/5nq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2H4A2Q2_9APHY A0A2H4A2Q2_9APHY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Laccases are enzymes that have the ability to catalyze the oxidation of a wide spectrum of phenolic compounds with the four-electron reduction of molecular oxygen to water. The active site of those proteins contains four copper ions, classified into three types. Laccases are interesting enzymes for study from the point of view of their structure, function and application because of their role in lignin degradation. Structural studies of two thermostable laccases produced by the strain Pycnoporus sanguineus CS43 (PsLacI and PsLacII) were performed. Both isoforms of PsLac show high thermal stability, at 60 degrees C and 50 degrees C, respectively, and they remained active at a high concentration of organic solvents. However, PsLacI has a higher thermal and pH stability and tolerance against inhibitors, and is a more efficient catalyst for ABTS and DMP (laccases substrate) than PsLacII. Based on the determined crystal structures we achieved insights into the structural factors relevant for the enzymatic properties of PsLacI and PsLacII. N-glycosylation site Asn354, which is very often present in structures of fungal laccases from other species, was not present in PsLac. This observation may be of particular significance due to the close distance between Asn354 and the substrate-binding pocket. This results in better access to the hydrophobic cavity for a particular substrate. Furthermore, we identified significant differences in the region of substrate-binding pocket, which confer PsLacI a markedly better performance than PsLacII.

Structural studies of two thermostable laccases from the white-rot fungus Pycnoporus sanguineus.,Orlikowska M, de J Rostro-Alanis M, Bujacz A, Hernandez-Luna C, Rubio R, Parra R, Bujacz G Int J Biol Macromol. 2017 Oct 18. pii: S0141-8130(17)32759-9. doi:, 10.1016/j.ijbiomac.2017.10.024. PMID:29055703<ref>PMID:29055703</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5nq9" style="background-color:#fffaf0;"></div>

==See Also==
*[[Laccase 3D structures|Laccase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trametes sanguinea]]
[[Category: Bujacz A]]
[[Category: Bujacz G]]
[[Category: Hernandez-Luna C]]
[[Category: Orlikowska M]]
[[Category: Parra R]]
[[Category: Rubio R]]
[[Category: De JRostro-Alanis M]]