View source for 5gxu


==Cystal structure of Arabidopsis ATR2==
<StructureSection load='5gxu' size='340' side='right'caption='[[5gxu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gxu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GXU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gxu OCA], [https://pdbe.org/5gxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gxu RCSB], [https://www.ebi.ac.uk/pdbsum/5gxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gxu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NCPR2_ARATH NCPR2_ARATH] This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and dichloroindophenol.[HAMAP-Rule:MF_03212]<ref>PMID:10733884</ref> <ref>PMID:9235908</ref> <ref>PMID:9449848</ref> <ref>PMID:9880378</ref> <ref>PMID:9990323</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Members of the cytochrome P450 family catalyze a variety of mono-oxygenase reactions, and for the eukaryotic membrane-bound members, NADPH is typically used as the reducing agent. The flavoprotein NADPH-cytochrome P450 reductase (CPR) enables electron transfer from NADPH to cytochrome P450 via its flavin cofactors. ATR2 is one of the two authentic CPR genes in the genome of the model plant Arabidopsis thaliana, and its product has been physiologically and kinetically characterized. Here, we report the 2.3 A structure of Arabidopsis thaliana NADPH-cytochrome P450 reductase 2 (ATR2) and find that the position of the two flavin cofactors differs from that of other known CPR structures. Mutation of residues related to possible interflavin electron transfer retains the reductase activity of ATR2, which suggests a direct electron transfer pathway between the flavins. In contrast, mutation of a single residue (R708) mediating interdomain interaction abolishes this activity. Because this residue is only conserved in plant CPRs, we speculate a plant-specific working mechanism as observed in ATR2. DATABASE: Atomic coordinates and structure factors of ATR2 are available in the Protein Data Bank under the accession code 5GXU.

Structure of the Arabidopsis thaliana NADPH-cytochrome P450 reductase 2 (ATR2) provides insight into its function.,Niu G, Zhao S, Wang L, Dong W, Liu L, He Y FEBS J. 2017 Mar;284(5):754-765. doi: 10.1111/febs.14017. Epub 2017 Feb 8. PMID:28103421<ref>PMID:28103421</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5gxu" style="background-color:#fffaf0;"></div>

==See Also==
*[[NADPH-Cytochrome P450 Reductase|NADPH-Cytochrome P450 Reductase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Liu L]]
[[Category: Niu G]]