View source for 5g2c


==The crystal structure of light-driven chloride pump ClR (T102D) mutant at pH 4.5.==
<StructureSection load='5g2c' size='340' side='right'caption='[[5g2c]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5g2c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nonlabens_marinus_S1-08 Nonlabens marinus S1-08]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G2C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g2c OCA], [https://pdbe.org/5g2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g2c RCSB], [https://www.ebi.ac.uk/pdbsum/5g2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g2c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/W8VZW3_9FLAO W8VZW3_9FLAO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A novel light-driven chloride-pumping rhodopsin (ClR) containing an 'NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08(T) determined under two conditions at 2.0 and 1.56 A resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a '3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif.,Kim K, Kwon SK, Jun SH, Cha JS, Kim H, Lee W, Kim JF, Cho HS Nat Commun. 2016 Aug 24;7:12677. doi: 10.1038/ncomms12677. PMID:27554809<ref>PMID:27554809</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5g2c" style="background-color:#fffaf0;"></div>

==See Also==
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nonlabens marinus S1-08]]
[[Category: Cha JS]]
[[Category: Cho HS]]
[[Category: Jun SH]]
[[Category: Kim HY]]
[[Category: Kim JH]]
[[Category: Kim KL]]
[[Category: Kwon SK]]