View source for 4y6t


==Structure of Tobacco streak virus coat protein dimer at 2.4 Angstroms resolution==
<StructureSection load='4y6t' size='340' side='right'caption='[[4y6t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4y6t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_streak_virus Tobacco streak virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y6T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y6t OCA], [https://pdbe.org/4y6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y6t RCSB], [https://www.ebi.ac.uk/pdbsum/4y6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y6t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A7UMQ4_9BROM A7UMQ4_9BROM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from protecting the viral genome, CPs of ilarviruses play several key roles in the life cycle of these viruses. Unlike the related bromo and cucumoviruses, ilarvirus particles are labile and pleomorphic, which has posed difficulties in their crystallization and structure determination. In the current study, a truncated TSV-CP was crystallized in two distinct forms and their structures were determined at resolutions of 2.4A and 2.1A, respectively. The core of TSV CP was found to possess the canonical beta-barrel jelly roll tertiary structure observed in several other viruses. Dimers of CP with swapped C-terminal arms (C-arm) were observed in both the crystal forms. The C-arm was found to be flexible and is likely to be responsible for the polymorphic and pleomorphic nature of TSV capsids. Consistent with this observation, mutations in the hinge region of the C-arm that reduce the flexibility resulted in the formation of more uniform particles. TSV CP was found to be structurally similar to that of Alfalfa mosaic virus (AMV) accounting for similar mechanism of genome activation in alfamo and ilar viruses. This communication represents the first report on the structure of the CP from an ilarvirus.

Structural studies on tobacco streak virus coat protein: Insights into the pleomorphic nature of ilarviruses.,Gulati A, Alapati K, Murthy A, Savithri HS, Murthy MR J Struct Biol. 2015 Dec 17. pii: S1047-8477(15)30113-1. doi:, 10.1016/j.jsb.2015.12.007. PMID:26706030<ref>PMID:26706030</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4y6t" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Tobacco streak virus]]
[[Category: Gulati A]]
[[Category: Murthy MRN]]