View source for 4xhv


==Crystal structure of Drosophila Spinophilin-PDZ and a C-terminal peptide of Neurexin==
<StructureSection load='4xhv' size='340' side='right'caption='[[4xhv]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xhv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XHV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.23&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xhv OCA], [https://pdbe.org/4xhv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xhv RCSB], [https://www.ebi.ac.uk/pdbsum/4xhv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xhv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M9NFI9_DROME M9NFI9_DROME] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis.

Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.,Muhammad K, Reddy-Alla S, Driller JH, Schreiner D, Rey U, Bohme MA, Hollmann C, Ramesh N, Depner H, Lutzkendorf J, Matkovic T, Gotz T, Bergeron DD, Schmoranzer J, Goettfert F, Holt M, Wahl MC, Hell SW, Scheiffele P, Walter AM, Loll B, Sigrist SJ Nat Commun. 2015 Oct 16;6:8362. doi: 10.1038/ncomms9362. PMID:26471740<ref>PMID:26471740</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xhv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Bergeron D]]
[[Category: Boehme MA]]
[[Category: Depner H]]
[[Category: Driller JH]]
[[Category: Goettfert F]]
[[Category: Hell SW]]
[[Category: Hollmann C]]
[[Category: Holt M]]
[[Category: Loll B]]
[[Category: Luetzkendorf J]]
[[Category: Matkovic T]]
[[Category: Muhammad KGH]]
[[Category: Quentin C]]
[[Category: Ramesh N]]
[[Category: Reddy S]]
[[Category: Rey U]]
[[Category: Schmoranzer J]]
[[Category: Sigrist SJ]]
[[Category: Wahl MC]]
[[Category: Walter A]]