View source for 4xbm


==X-ray crystal structure of Notch ligand Delta-like 1==
<StructureSection load='4xbm' size='340' side='right'caption='[[4xbm]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xbm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XBM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xbm OCA], [https://pdbe.org/4xbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xbm RCSB], [https://www.ebi.ac.uk/pdbsum/4xbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xbm ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/DLL1_HUMAN DLL1_HUMAN] Septopreoptic holoprosencephaly;Semilobar holoprosencephaly;Lobar holoprosencephaly;Alobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Microform holoprosencephaly. 
== Function ==
[https://www.uniprot.org/uniprot/DLL1_HUMAN DLL1_HUMAN] Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.<ref>PMID:11581320</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Notch pathway is a fundamental signalling system in most multicellular animals. We have determined the X-ray crystal structure of the extracellular domain of the Notch ligand Delta-like ligand-1 (Dll-1). This is the first structure of a Delta-like ligand. The structure incorporates the N-terminal C2 domain, receptor-binding DSL domain, and the first six (of eight) EGF-like repeats, which form a highly extended conformation, confirmed by analytical ultracentrifugation. Comparison of our structure with a fragment of Jagged1 ligand allows us to dissect the similarities and differences between the ligand families. Differences in the C2 domains of Dll-1 and Jagged1 suggest their lipid binding properties are likely to differ. A conserved hydrophobic patch on the surface of both Dll-1 and Jagged1 provides a likely receptor-interaction site that is common to both ligands. We also explore the binding affinity of Dll-1 for a fragment of Notch1 using different techniques. Apparent binding affinities vary when different techniques are used, explaining discrepancies in the literature. Using analytical ultracentrifugation, we perform for the first time binding analyses where both receptor and ligand are in solution, which confirms a Kd of 10muM for this interaction.

Notch ligand Delta-like1: X-ray crystal structure and binding affinity.,Kershaw NJ, Church NL, Griffin MD, Luo CS, Adams TE, Burgess AW Biochem J. 2015 Feb 26. PMID:25715738<ref>PMID:25715738</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xbm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Adam TE]]
[[Category: Burgess AW]]
[[Category: Church NL]]
[[Category: Kershaw NJ]]
[[Category: Luo CS]]