View source for 4mct


==P. vulgaris HIGBA structure, crystal form 1==
<StructureSection load='4mct' size='340' side='right'caption='[[4mct]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mct]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MCT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mct OCA], [https://pdbe.org/4mct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mct RCSB], [https://www.ebi.ac.uk/pdbsum/4mct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mct ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HIGA_PROVU HIGA_PROVU] Antitoxin component of a type II toxin-antitoxin (TA) system that counteracts the effect of the HigB toxin (PubMed:19423702, PubMed:8645296, PubMed:24257752). Binds to its own promoter and regulates transcription of the higB/higA operon (PubMed:24257752).<ref>PMID:19423702</ref> <ref>PMID:24257752</ref> <ref>PMID:8645296</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial toxin-antitoxin (TA) systems regulate key cellular processes to promote cell survival during periods of stress. During steady-state cell growth, antitoxins typically interact with their cognate toxins to inhibit activity presumably by preventing substrate recognition. We solved two X-ray crystal structures of the Proteus vulgaris tetrameric HigB-(HigA)2-HigB TA complex and find that, unlike most other TA systems, the antitoxin HigA makes minimal interactions with the toxin HigB. HigB adopts a RelE-family tertiary fold containing a highly conserved, concave surface where we predict its active site is located. HigA does not cover the solvent exposed HigB active site, suggesting that in general, toxin inhibition is not solely mediated by active site hindrance by antitoxin binding. Each HigA monomer contains a helix-turn-helix (HTH) motif that binds to its own DNA operator to repress transcription during normal cellular growth. This is distinct from antitoxins belonging to other super families that typically only form DNA-binding motifs upon dimerization. We further show that disruption of the HigB-(HigA)2-HigB tetramer to a HigBA heterodimer ablates operator binding. Taken together, our biochemical and structural studies elucidate the novel molecular details of the HigBA TA system.

Structure of the P. vulgaris HigB-(HigA)2-HigB toxin-antitoxin complex.,Schureck MA, Maehigashi T, Miles SJ, Marquez J, Ei Cho S, Erdman R, Dunham CM J Biol Chem. 2013 Nov 20. PMID:24257752<ref>PMID:24257752</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4mct" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Proteus vulgaris]]
[[Category: Dunham CM]]
[[Category: Maehigashi T]]
[[Category: Schureck MA]]