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==Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens.==
<StructureSection load='4afm' size='340' side='right'caption='[[4afm]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4afm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_cellulosolvens Eubacterium cellulosolvens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AFM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4afm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4afm OCA], [https://pdbe.org/4afm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4afm RCSB], [https://www.ebi.ac.uk/pdbsum/4afm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4afm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q3LHN3_EUBCE Q3LHN3_EUBCE] 
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== Publication Abstract from PubMed ==
Plant biomass is central to the carbon cycle and to environmentally sustainable industries exemplified by the biofuel sector. Plant cell wall degrading enzymes generally contain non-catalytic carbohydrate binding modules (CBMs) that fulfil a targeting function, which enhances catalysis. CBMs that bind beta-glucan chains often display broad specificity recognizing beta-1,4-glucans (cellulose), beta-1,3-beta-1,4-mixed linked glucans and xyloglucan, a beta-1,4-glucan decorated with alpha-1,6-xylose residues, by targeting structures common to the three polysaccharides. Thus, CBMs that recognize xyloglucan target the beta-1,4-glucan backbone and only accommodate the xylose decorations. Here we show that two closely related CBMs, CBM65A and CBM65B, derived from EcCel5A, a Eubacterium cellulosolvens endoglucanase, bind to a range of beta-glucans but, uniquely, display significant preference for xyloglucan. The structures of the two CBMs reveal a beta-sandwich fold. The ligand binding site comprises the beta-sheet that forms the concave surface of the proteins. Binding to the backbone chains of beta-glucans is mediated primarily by five aromatic residues that also make hydrophobic interactions with the xylose side chains of xyloglucan, conferring the distinctive specificity of the CBMs for the decorated polysaccharide. Significantly, and in contrast to other CBMs that recognize beta-glucans, CBM65A utilizes different polar residues to bind cellulose and mixed linked glucans. Thus, Gln106 is central to cellulose recognition, but is not required for binding to mixed linked glucans. This report reveals the mechanism by which beta-glucan-specific CBMs can distinguish between linear and mixed linked glucans, and show how these CBMs can exploit an extensive hydrophobic platform to target the side chains of decorated beta-glucans.

Understanding how non-catalytic carbohydrate binding modules can display specificity for xyloglucan.,Luis AS, Venditto I, Prates JA, Ferrieira LM, Temple MJ, Rogowski A, Basle A, Xue J, Knox JP, Najmudin S, Fontes CM, Gilbert HJ J Biol Chem. 2012 Dec 10. PMID:23229556<ref>PMID:23229556</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4afm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ferreira LMA]]
[[Category: Fontes CMGA]]
[[Category: Gilbert HJ]]
[[Category: Luis AS]]
[[Category: Najmudin S]]
[[Category: Prates JAM]]
[[Category: Venditto I]]