View source for 3go3


==Interactions of an echinomycin-DNA complex with manganese(II) ions==
<StructureSection load='3go3' size='340' side='right'caption='[[3go3]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3go3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_echinatus Streptomyces echinatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GO3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=N2C:N,S-DIMETHYLCYSTEINE'>N2C</scene>, <scene name='pdbligand=NCY:N-METHYLCYSTEINE'>NCY</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[185d|185d]], [[193d|193d]], [[1pfe|1pfe]], [[1vs2|1vs2]], [[1xvk|1xvk]], [[1xvn|1xvn]], [[1xvr|1xvr]], [[2adw|2adw]], [[2da8|2da8]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3go3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3go3 OCA], [https://pdbe.org/3go3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3go3 RCSB], [https://www.ebi.ac.uk/pdbsum/3go3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3go3 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of an echinomycin-d(ACGTACGT) duplex interacting with manganese(II) was solved by Mn-SAD using in-house data and refined to 1.1 A resolution against synchrotron data. This complex crystallizes in a different space group compared with related complexes and shows a different mode of base pairing next to the bis-intercalation site, suggesting that the energy difference between Hoogsteen and Watson-Crick pairing is rather small. The binding of manganese to N7 of guanine is only possible because of DNA unwinding induced by the echinomycin, which might help to explain the mode of action of the drug.

Interaction of an echinomycin-DNA complex with manganese ions.,Pfoh R, Cuesta-Seijo JA, Sheldrick GM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt, 7):660-4. Epub 2009 Jun 27. PMID:19574634<ref>PMID:19574634</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3go3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces echinatus]]
[[Category: Cuesta-Seijo, J A]]
[[Category: Pfoh, R]]
[[Category: Sheldrick, G M]]
[[Category: Antibiotic]]
[[Category: Antitumor]]
[[Category: Bisintercalator]]
[[Category: Depsipeptide]]
[[Category: Dna-antibiotic complex]]
[[Category: Hoogsteen basepair]]
[[Category: Quinoxaline]]
[[Category: Thioacetal]]