View source for 3aiz


==Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (P21212)==
<StructureSection load='3aiz' size='340' side='right'caption='[[3aiz]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3aiz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aiz OCA], [https://pdbe.org/3aiz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aiz RCSB], [https://www.ebi.ac.uk/pdbsum/3aiz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aiz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCNA2_SULTO PCNA2_SULTO] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication (By similarity). Both trimeric complexes inhibit DNA ligase and both 3'-5' and 5'-3' activity of Hel308 (Hjm) helicase, but stimulate Hjc, the Holliday junction cleavage enzyme.[HAMAP-Rule:MF_00317]<ref>PMID:18782564</ref> 
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== Publication Abstract from PubMed ==
Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2-stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2-stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.

A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex.,Kawai A, Hashimoto H, Higuchi S, Tsunoda M, Sato M, Nakamura KT, Miyamoto S J Struct Biol. 2011 Jun;174(3):443-50. Epub 2011 Feb 23. PMID:21352919<ref>PMID:21352919</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3aiz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfurisphaera tokodaii str. 7]]
[[Category: Higuchi S]]
[[Category: Kawai A]]
[[Category: Miyamoto S]]