View source for 2keq


==Solution structure of DnaE intein from Nostoc punctiforme==
<StructureSection load='2keq' size='340' side='right'caption='[[2keq]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2keq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KEQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2keq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2keq OCA], [https://pdbe.org/2keq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2keq RCSB], [https://www.ebi.ac.uk/pdbsum/2keq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2keq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2J066_NOSP7 B2J066_NOSP7] [https://www.uniprot.org/uniprot/B2J821_NOSP7 B2J821_NOSP7] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/2keq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2keq ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated.

Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification.,Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H FEBS Lett. 2009 May 6;583(9):1451-6. Epub 2009 Apr 1. PMID:19344715<ref>PMID:19344715</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2keq" style="background-color:#fffaf0;"></div>

==See Also==
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nostoc punctiforme PCC 73102]]
[[Category: Aranko AS]]
[[Category: Djupsj JB]]
[[Category: Iwai H]]
[[Category: Oeemig JS]]