View source for 1mxp


==Solution structure of the ribbon disulfide bond isomer of alpha-conotoxin AuIB==
<StructureSection load='1mxp' size='340' side='right'caption='[[1mxp]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MXP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mxp OCA], [https://pdbe.org/1mxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mxp RCSB], [https://www.ebi.ac.uk/pdbsum/1mxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mxp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
alpha-Conotoxin AuIB and a disulfide bond variant of AuIB have been synthesized to determine the role of disulfide bond connectivity on structure and activity. Both of these peptides contain the 15 amino acid sequence GCCSYPPCFATNPDC, with the globular (native) isomer having the disulfide connectivity Cys(2-8 and 3-15) and the ribbon isomer having the disulfide connectivity Cys(2-15 and 3-8). The solution structures of the peptides were determined by NMR spectroscopy, and their ability to block the nicotinic acetylcholine receptors on dissociated neurons of the rat parasympathetic ganglia was examined. The ribbon disulfide isomer, although having a less well defined structure, is surprisingly found to have approximately 10 times greater potency than the native peptide. To our knowledge this is the first demonstration of a non-native disulfide bond isomer of a conotoxin exhibiting greater biological activity than the native isomer.

A new level of conotoxin diversity, a non-native disulfide bond connectivity in alpha-conotoxin AuIB reduces structural definition but increases biological activity.,Dutton JL, Bansal PS, Hogg RC, Adams DJ, Alewood PF, Craik DJ J Biol Chem. 2002 Dec 13;277(50):48849-57. Epub 2002 Oct 9. PMID:12376538<ref>PMID:12376538</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1mxp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Conus aulicus]]
[[Category: Large Structures]]
[[Category: Adams DJ]]
[[Category: Alewood PF]]
[[Category: Bansal PS]]
[[Category: Craik DJ]]
[[Category: Dutton JL]]
[[Category: Hogg RC]]