View source for User:Mary Ball/AFP

{{Seed}}
[[Image:1wfb.png|left|200px]]

<!--
The line below this paragraph, containing "STRUCTURE_1wfb", creates the "Structure Box" on the page.
You may change the PDB parameter (which sets the PDB file loaded into the applet) 
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
or leave the SCENE parameter empty for the default display.
-->
{{STRUCTURE_1wfb|  PDB=1wfb  |  SCENE=  }} 

===WINTER FLOUNDER ANTIFREEZE PROTEIN===
Type I antifreeze proteins (AFPs) are grouped together according to similar structure.  The one shown on the right is from the winter flounder. 
This AFP consists of a chain of 37 amino acids: DTASDAAAAAALTAANAKAAAELTAANAAAAAAATAR  The chain forms a single alpha-helix. 

The four threonines are evenly spaced in the chain, such that there are three 11-amino-acid sequences.  In the single alpha-helix that forms, the threonines all lie on one "face" of the helix.

<scene name='User:Mary_Ball/AFP/Threonine_residues/3'>See the location of the Threonine Residues.</scene>

<scene name='User:Mary_Ball/AFP/Amino_acids/1'>See the Threonine Residues.</scene>

<scene name='User:Mary_Ball/AFP/Amino_acids/2'>See one chain emphasized.</scene>
 
<scene name='User:Mary_Ball/Sandbox/Wfb/1'>Go back to the WFB Alpha Helix.</scene>

Baardsnes, et al (1999) created mutations that reduced the protein's ice-binding ability. They also compared the sequences of five different type I AFP molecules. They concluded that the "face" with the repeated Threonines promotes binding to ice crystals.

===REFERENCE===

New ice-binding face for type I antifreeze protein., Baardsnes J, Kondejewski LH, Hodges RS, Chao H, Kay C, Davies PL, FEBS Lett. 1999 Dec 10;463(1-2):87-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10601644 10601644]



<!-- 
The line below this paragraph, {{ABSTRACT_PUBMED_10601644}}, adds the Publication Abstract to the page 
(as it appears on PubMed at http://www.pubmed.gov), where 10601644 is the PubMed ID number.
-->

==ABOUT THIS STRUCTURE==
1WFB is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Pseudopleuronectes_americanus Pseudopleuronectes americanus]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WFB OCA]. 

==REFERENCE==
<ref group="xtra">PMID:7760940</ref><references group="xtra"/>
[[Category: Antifreeze Proteins]]
[[Category: Pseudopleuronectes americanus]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Sicheri, F.]]
[[Category: Yang, D S.C.]]
[[Category: Antifreeze polypeptide]]
[[Category: Ice binding protein]]
[[Category: Thermal hysteresis protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 08:56:47 2010''

===WINTER FLOUNDER ANTIFREEZE PROTEIN ISOFORM HPLC6 AT-180 DEGREES C===


<!-- 
The line below this paragraph, {{ABSTRACT_PUBMED_7760940}}, adds the Publication Abstract to the page 
(as it appears on PubMed at http://www.pubmed.gov), where 7760940 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_7760940}}