View source for 8pay


==Structure of the E.coli DNA polymerase sliding clamp with a covalently bound peptide 2.==
<StructureSection load='8pay' size='340' side='right'caption='[[8pay]], [[Resolution|resolution]] 1.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8pay]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PAY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ALC:2-AMINO-3-CYCLOHEXYL-PROPIONIC+ACID'>ALC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LV8:(2~{S})-3-acetamido-2-azanyl-propanoic+acid'>LV8</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pay OCA], [https://pdbe.org/8pay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pay RCSB], [https://www.ebi.ac.uk/pdbsum/8pay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pay ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C3SLM2_ECOLX C3SLM2_ECOLX] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Blagojevic J]]
[[Category: Brillet K]]
[[Category: Burnouf D]]
[[Category: Compain G]]
[[Category: Dumas P]]
[[Category: Engilberge S]]
[[Category: Guichard G]]
[[Category: Hammann P]]
[[Category: Kuhn L]]
[[Category: Martiel I]]
[[Category: Monsarrat C]]
[[Category: Olieric V]]
[[Category: Wolff P]]
[[Category: Wagner J]]