View source for 8c8d


==Crystal structure of human DNA cross-link repair 1A in complex with hydroxamic acid inhibitor (compound 44).==
<StructureSection load='8c8d' size='340' side='right'caption='[[8c8d]], [[Resolution|resolution]] 1.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8c8d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C8D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c8d OCA], [https://pdbe.org/8c8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c8d RCSB], [https://www.ebi.ac.uk/pdbsum/8c8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c8d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCR1A_HUMAN DCR1A_HUMAN] May be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons.<ref>PMID:15542852</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three human SNM1 metallo-beta-lactamase fold nucleases (SNM1A-C) play key roles in DNA damage repair and in maintaining telomere integrity. Genetic studies indicate that they are attractive targets for cancer treatment and to potentiate chemo- and radiation-therapy. A high-throughput screen for SNM1A inhibitors identified diverse pharmacophores, some of which were shown by crystallography to coordinate to the di-metal ion centre at the SNM1A active site. Structure and turnover assay-guided optimization enabled the identification of potent quinazoline-hydroxamic acid containing inhibitors, which bind in a manner where the hydroxamic acid displaces the hydrolytic water and the quinazoline ring occupies a substrate nucleobase binding site. Cellular assays reveal that SNM1A inhibitors cause sensitisation to, and defects in the resolution of, cisplatin-induced DNA damage, validating the tractability of MBL fold nucleases as cancer drug targets.

Cell-active small molecule inhibitors validate the SNM1A DNA repair nuclease as a cancer target.,Bielinski M, Henderson LR, Yosaatmadja Y, Swift LP, Baddock HT, Bowen MJ, Brem J, Jones PS, McElroy SP, Morrison A, Speake M, van Boeckel S, van Doornmalen E, van Groningen J, van den Hurk H, Gileadi O, Newman JA, McHugh PJ, Schofield CJ Chem Sci. 2024 Apr 30;15(21):8227-8241. doi: 10.1039/d4sc00367e. eCollection 2024 , May 29. PMID:38817593<ref>PMID:38817593</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8c8d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Baddock HT]]
[[Category: Bielinski M]]
[[Category: Bountra C]]
[[Category: Gileadi O]]
[[Category: McHugh PJ]]
[[Category: Newman JA]]
[[Category: Schofield CJ]]
[[Category: Yosaatmadja Y]]
[[Category: Von Delft F]]