View source for 7xm1


==Cryo-EM structure of mTIP60-Ba (metal-ion induced TIP60 (K67E) complex with barium ions==
<StructureSection load='7xm1' size='340' side='right'caption='[[7xm1]], [[Resolution|resolution]] 3.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xm1]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XM1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.96&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xm1 OCA], [https://pdbe.org/7xm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xm1 RCSB], [https://www.ebi.ac.uk/pdbsum/7xm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xm1 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein nanocages are of increasing interest for use as drug capsules, but the encapsulation and release of drug molecules at appropriate times require the reversible association and dissociation of the nanocages. One promising approach to addressing this challenge is the design of metal-dependent associating proteins. Such designed proteins typically have Cys or His residues at the protein surface for connecting the associating proteins through metal-ion coordination. However, Cys and His residues favor interactions with soft and borderline metal ions, such as Au(+) and Zn(2+), classified by the hard and soft acids and bases concept, restricting the types of metal ions available to drive association. Here, we show the alkaline earth (AE) metal-dependent association of the recently designed artificial protein nanocage TIP60, which is composed of 60-mer fusion proteins. The introduction of a Glu (hard base) mutation to the fusion protein (K67E mutant) prevented the formation of the 60-mer but formed the expected cage structure in the presence of Ca, Sr, or Ba ions (hard acids). Cryogenic electron microscopy (cryo-EM) analysis indicated a Ba ion at the interface of the subunits. Furthermore, we demonstrated the encapsulation and release of single-stranded DNA molecules using this system. Our results provide insights into the design of AE metal-dependent association and dissociation mechanisms for proteins.

Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions.,Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K J Am Chem Soc. 2023 Jan 11;145(1):216-223. doi: 10.1021/jacs.2c09537. Epub 2022 , Dec 21. PMID:36541447<ref>PMID:36541447</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xm1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Adachi N]]
[[Category: Arai R]]
[[Category: Kawakami N]]
[[Category: Kawasaki M]]
[[Category: Miyamoto K]]
[[Category: Moriya T]]
[[Category: Ohara N]]