View source for 7wr3


==Crystal structure of MBP-fused OspC3 in complex with calmodulin==
<StructureSection load='7wr3' size='340' side='right'caption='[[7wr3]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wr3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bolitoglossa Bolitoglossa], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WR3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wr3 OCA], [https://pdbe.org/7wr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wr3 RCSB], [https://www.ebi.ac.uk/pdbsum/7wr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wr3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/OSPC3_SHIFL OSPC3_SHIFL] ADP-riboxanase effector that inhibits host cell pyroptosis (PubMed:23684308, PubMed:34409271, PubMed:34671164). Acts by mediating arginine ADP-riboxanation of host CASP4/CASP11, blocking CASP4/CASP11 autoprocessing (PubMed:34671164, PubMed:35338844, PubMed:35568036). This prevents CASP4 activation and ability to recognize and cleave GSDMD, thereby inhibiting LPS-induced pyroptosis (PubMed:34671164). ADP-riboxanation takes place in two steps: OspC3 first catalyzes ADP-ribosylation of target Arg, and then initiates a deamination to remove one N-omega group (PubMed:34671164). Independently of its ADP-riboxanase activity, acts as an inhibitor of calcium signaling by inhibiting host calmodulin, preventing activation of the JAK-STAT signaling pathway in response to interferon-beta (PubMed:35568036). Mechanistically, acts by binding to the apo form of calmodulin, preventing calcium-binding and ability to activate host CaMK2 (CAMKII), which is required to stimulate the JAK-STAT signaling pathway in response to interferon-beta (PubMed:35568036).<ref>PMID:23684308</ref> <ref>PMID:34409271</ref> <ref>PMID:34671164</ref> <ref>PMID:35338844</ref> <ref>PMID:35568036</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bolitoglossa]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Shigella flexneri]]
[[Category: Ding J]]
[[Category: Hou YJ]]
[[Category: Shao F]]
[[Category: Zeng H]]