View source for 7uwa


==Citrus V-ATPase State 1, H in contact with subunits AB==
<StructureSection load='7uwa' size='340' side='right'caption='[[7uwa]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7uwa]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_limon Citrus limon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UWA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uwa OCA], [https://pdbe.org/7uwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uwa RCSB], [https://www.ebi.ac.uk/pdbsum/7uwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uwa ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
We used the Legionella pneumophila effector SidK to affinity purify the endogenous vacuolar-type ATPases (V-ATPases) from lemon fruit. The preparation was sufficient for cryoelectron microscopy, allowing structure determination of the enzyme in two rotational states. The structure defines the ATP:H(+) ratio of the enzyme, demonstrating that it can establish a maximum DeltapH of approximately 3, which is insufficient to maintain the low pH observed in the vacuoles of juice sac cells in lemons and other citrus fruit. Compared with yeast and mammalian enzymes, the membrane region of the plant V-ATPase lacks subunit f and possesses an unusual configuration of transmembrane alpha helices. Subunit H, which inhibits ATP hydrolysis in the isolated catalytic region of V-ATPase, adopts two different conformations in the intact complex, hinting at a role in modulating activity in the intact enzyme.

Structure of V-ATPase from citrus fruit.,Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL Structure. 2022 Oct 6;30(10):1403-1410.e4. doi: 10.1016/j.str.2022.07.006. Epub , 2022 Aug 29. PMID:36041457<ref>PMID:36041457</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7uwa" style="background-color:#fffaf0;"></div>

==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Citrus limon]]
[[Category: Large Structures]]
[[Category: Abdelaziz RA]]
[[Category: Keon KA]]
[[Category: Rubinstein JL]]
[[Category: Schulze WX]]
[[Category: Schumacher K]]