View source for 7sd2


==Murine Fab that recognizes Hev b 8 (profilin for Hevea brasiliensis)==
<StructureSection load='7sd2' size='340' side='right'caption='[[7sd2]], [[Resolution|resolution]] 3.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7sd2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SD2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd2 OCA], [https://pdbe.org/7sd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd2 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd2 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Allergies have become a rising health problem, where plentiful substances can trigger IgE-mediated allergies in humans. While profilins are considered minor allergens, these ubiquitous proteins are primary molecules involved in cross-reactivity and pollen-food allergy syndrome. Here we report the first crystal structures of murine Fab/IgE, with its chains naturally paired, in complex with the allergen profilin from Hevea brasiliensis (Hev b 8). The crystallographic models revealed that the IgE's six complementarity-determining regions (CDRs) interact with the allergen, comprising a rigid paratope-epitope surface of 926 A(2), which includes an extensive network of interactions. Interestingly, we also observed previously unreported flexibility at Fab/IgE's elbow angle, which did not influence the shape of the paratope. The Fab/IgE exhibits a high affinity for Hev b 8, even when using 1 M NaCl in BLI experiments. Finally, based on the encouraging cross-reactivity assays using two mutants of the maize profilin (Zea m 12), this antibody could be a promising tool in IgE engineering for diagnosis and research applications.

A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity.,Garcia-Ramirez B, Mares-Mejia I, Rodriguez-Hernandez A, Cano-Sanchez P, Torres-Larios A, Ortega E, Rodriguez-Romero A Commun Biol. 2022 Jul 27;5(1):748. doi: 10.1038/s42003-022-03718-w. PMID:35902770<ref>PMID:35902770</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7sd2" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Garcia-Ramirez B]]
[[Category: Rodriguez-Romero A]]