View source for 7pqh


==Cryo-EM structure of Saccharomyces cerevisiae TOROID (TORC1 Organized in Inhibited Domains).==
<StructureSection load='7pqh' size='340' side='right'caption='[[7pqh]], [[Resolution|resolution]] 3.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7pqh]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PQH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.87&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pqh OCA], [https://pdbe.org/7pqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pqh RCSB], [https://www.ebi.ac.uk/pdbsum/7pqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pqh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KOG1_YEAST KOG1_YEAST] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Target of rapamycin complex 1 (TORC1) is a protein kinase controlling cell homeostasis and growth in response to nutrients and stresses. In Saccharomyces cerevisiae, glucose depletion triggers a redistribution of TORC1 from a dispersed localization over the vacuole surface into a large, inactive condensate called TOROID (TORC1 organized in inhibited domains). However, the mechanisms governing this transition have been unclear. Here, we show that acute depletion and repletion of EGO complex (EGOC) activity is sufficient to control TOROID distribution, independently of other nutrient-signaling pathways. The 3.9-A-resolution structure of TORC1 from TOROID cryo-EM data together with interrogation of key interactions in vivo provide structural insights into TORC1-TORC1' and TORC1-EGOC interaction interfaces. These data support a model in which glucose-dependent activation of EGOC triggers binding to TORC1 at an interface required for TOROID assembly, preventing TORC1 polymerization and promoting release of active TORC1.

EGOC inhibits TOROID polymerization by structurally activating TORC1.,Prouteau M, Bourgoint C, Felix J, Bonadei L, Sadian Y, Gabus C, Savvides SN, Gutsche I, Desfosses A, Loewith R Nat Struct Mol Biol. 2023 Mar;30(3):273-285. doi: 10.1038/s41594-022-00912-6. , Epub 2023 Jan 26. PMID:36702972<ref>PMID:36702972</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7pqh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bonadei L]]
[[Category: Bourgoint C]]
[[Category: Desfosses A]]
[[Category: Felix J]]
[[Category: Gabus C]]
[[Category: Gutsche I]]
[[Category: Loewith R]]
[[Category: Prouteau M]]
[[Category: Sadian Y]]
[[Category: Savvides SN]]