View source for 7och


==Apo-structure of Lassa virus L protein (well-resolved polymerase core) [APO-CORE]==
<StructureSection load='7och' size='340' side='right'caption='[[7och]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7och]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_mammarenavirus Lassa mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OCH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.14&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7och FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7och OCA], [https://pdbe.org/7och PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7och RCSB], [https://www.ebi.ac.uk/pdbsum/7och PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7och ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3S8NV63_LASV A0A3S8NV63_LASV] RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.[HAMAP-Rule:MF_04086][PIRNR:PIRNR000836]

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lassa mammarenavirus]]
[[Category: Busch C]]
[[Category: Cusack S]]
[[Category: Grunewald K]]
[[Category: Gunther S]]
[[Category: Kouba T]]
[[Category: Milewski M]]
[[Category: Quemin E]]
[[Category: Rosenthal M]]
[[Category: Thorkelsson S]]
[[Category: Vogel D]]
[[Category: William H]]